Coding
Part:BBa_K3589106:Design
Designed by: Yannik Schermer Group: iGEM20_TU_Kaiserslautern (2020-10-23)
Wildtype laccase from uncultured marine bacteria for Escherichia coli
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 517
Illegal PstI site found at 22
Illegal PstI site found at 52 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 517
Illegal PstI site found at 22
Illegal PstI site found at 52 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 517
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 517
Illegal PstI site found at 22
Illegal PstI site found at 52 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 517
Illegal PstI site found at 22
Illegal PstI site found at 52
Illegal NgoMIV site found at 840
Illegal AgeI site found at 93
Illegal AgeI site found at 1105 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The natural secretion signal of Botrytis aclada was removed (?). This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part originates from Botrytis aclada (Scheiblbrandner, S., 2017) and is condon-optimized for Chlamydomonas reinhardtii.