Coding

Part:BBa_K3589106:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-23)


Wildtype laccase from uncultured marine bacteria for Escherichia coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 517
    Illegal PstI site found at 22
    Illegal PstI site found at 52
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 517
    Illegal PstI site found at 22
    Illegal PstI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 517
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 517
    Illegal PstI site found at 22
    Illegal PstI site found at 52
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 517
    Illegal PstI site found at 22
    Illegal PstI site found at 52
    Illegal NgoMIV site found at 840
    Illegal AgeI site found at 93
    Illegal AgeI site found at 1105
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural secretion signal of Botrytis aclada was removed (?). This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part originates from Botrytis aclada (Scheiblbrandner, S., 2017) and is condon-optimized for Chlamydomonas reinhardtii.

References